资 源 简 介
Function
Basic Melting Temperature (Tm) Calculations for FASTA file;
Reference
http://www.biophp.org/minitools/melting_temperature/demo.php?formula=basic
Example
perl TM.Calculate.pl input.fa output.txt
input.fa
ENSE00001365742_single170_0|2006-05-16A|2006-05-16B/D
ATCGCACCAGCGTGTATGATCAGCACAGCCAGGGTACCTGCTGACAAGCCTGTACGCATCGCCTTTAGCCTCAATGACGCCTCAGGTAT
ACTCTGCTCTGGAAGAAGGATTGTTTCCTTCATACCCCACCTTTCTAGCATCTTTTCTAATGCCACGCCTCTGTGGAAAATTTAAAGTT
GCATGCTCACTGCGGCTCCTCA
ENSE00001408880_single170_0|2006-05-16A|2006-05-16B/D
ATCGCACCAGCGTGTATGATACACCCCCTGAAGACTCCATTCCTTTGGTCTTTCCAGAATTAGACCAGCAGCTACAGGTAAGGATTTTT
CTAAAGTCTCTCTTCTTTCCTCCTTAGTGTCATAAAGTATAGCGAGCTGGGCAGAGAGTGGCCTGACTAGGGGAGACTGACTTGGACCC
output.txt
ENSE00001365742_single170_0|2006-05-16A|2006-05-16B/D 82.038 ATCGCACCAGCGTGTA
